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KMID : 1134820150440081128
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Journal of the Korean Society of Food Science and Nutrition
2015 Volume.44 No. 8 p.1128 ~ p.1136
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Anti-Inflammatory Effect of Ethanol Extract from Grateloupia elliptica Holmes on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears
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Bae Nan-Young
Kim Min-Ji
Kim Koth-Bong-Woo-Ri
Ahn Na-Kyung
Choi Yeon-Uk
Park Ji-Hye
Park Sun-Hee
Ahn Dong-Hyun
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Abstract
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This study investigated the anti-inflammatory effects of ethanol extract from Grateloupia elliptica Holmes (GEHEE) on the lipopolysaccharide-induced inflammatory response. Anti-inflammatory effects were detected by enzyme-linked immunosorbent assay, Western blotting, and immunohistochemistry. There were no cytotoxic effects on proliferation of macrophages treated with GEHEE compared to the control. GEHEE remarkably suppressed NO and pro-inflammatory cytokines (interleukin-6, tumor necrosis factor-α, and interleukin-1β) production and reduced expression of inducible nitric oxide synthase, cyclooxygenase-2, and nuclear factor-κB (NF-κB) proteins in a dose-dependent manner. GEHEE also significantly reduced activation of mitogen-activated protein kinases (MAPKs). The formation of edema in mouse ears was reduced at the highest dose compared to the control. GEHEE also reduced dermal thickness and mast cell numbers based on histological analysis. These results suggest that GEHEE exerts significant anti-inflammatory activity via inhibition of NF-κB and MAPKs activation and may be a potential anti-inflammatory therapeutic material.
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KEYWORD
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Grateloupia elliptica Holmes, anti-inflammatory activity, NF-κB, MAPKs, ear edema
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